|
KMID : 0616120080390020095
|
|
Korean Journal of Pharmacognosy
2008 Volume.39 No. 2 p.95 ~ p.103
|
|
|
Inhibition of LPS induced iNOS, COX-2 and cytokines expression by kaempferol-3-O-β-D-sophoroside through the NF-κB inactivation in RAW 264.7 cells
|
|
Park Seung-Jae
Lee Kyung-Tae
Baek Nam-In
Ahn Eun-Mi
Cho Young-Wuk
Shin Ji-Sun
Cho Woong
|
|
|
Abstract
|
|
|
In the present study, we investigated the anti-inflammatory effects by kaempferol-3-O-β-D-sophoroside (KS) isolated from Sophora japonica (Leguminosae) on the lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin (PGE2) production by RAW 264.7 cell line compared with kaempferol. KS significantly inhibited the LPS-induced NO and PGE2 production. Consistent with these observations, KS reduced the LPS-induced expression of inducible nitric oxide synthase
(iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels in a concentration-dependent manner. In addition, the release and the mRNA expression levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were also reduced by KS. Moreover, KS attenuated the LPS-induced activation of nuclear factor-kappa B (NF-κB), a transcription factor necessary for pro-inflammatory mediators, iNOS, COX-2, TNF-α and IL-6 expression. These results suggest that the down regulation of iNOS, COX-2, TNF-α, and IL-6 expression by KS are achieved by the downregulation of NF-κB activity, and that is also responsible for its anti-inflammatory effects.
|
|
|
KEYWORD
|
|
|
Kaempferol-3-O-β-D-sophoroside, NF-κB, LPS, Anti-inflammation, Sophora japonica
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|